Tailoring surface properties of functionalized graphene papers aiming to enzyme immobilization

The use of enzymes as catalysts requires recovery and reuse to make the process viable. Enzymatic immobilization changes enzyme stability, activity, specificity. It is very important explore new substrates for with appropriate composition structure improve efficiency immobilized enzymes. This work explores two different graphene oxide papers, one produced by oxidation route (GO) other electrochemical synthesis (EG), aiming ?-galactosidase immobilization. chemical structural properties these papers were characterized Raman spectroscopy, X-ray photoelectron spectroscopy diffraction. Atomic force microscopy images showed that EG paper ensured more efficient on surface paper. Cyclic voltammetry was used monitor reaction conversion lactose glucose in free solution solutions. cyclic analysis GO an improvement activity ?-galactose when compared solution, well a glassy carbon electrode.